References and Literature

The concept for a Pneumocystis carinii (Pc) Genome Project was presented to the community at the 5th International Workshop on Pneumocystis held in Lille, France, September, 1997 (Cushion MT and Arnold JA. 1998. Proposal for a Pneumocystis Genome project. J. Euk. Microbiol. 44: 7s.). There, consensus for such a project, the approach to be used, the genome(s) of focus, and the reagents to be generated were decided upon.

The consensus of the community was to embark on a Pc Genome project using a physical mapping strategy followed by directed sequencing. The cosmid libraries created would become a resource to the scientific community. It was also agreed to begin the project using the rat Form 1 P. carinii.

Descriptions of the initiation and plans
for the Pneumocystis Genome Project

Cushion MT, Arnold J. Proposal for a Pneumocystis genome project.
J Eukaryot Microbiol. 1997 Nov-Dec;44(6):7S.

Arnold J, Cushion MT. Constructing a physical map of the Pneumocystis genome.
J Eukaryot Microbiol. 1997 Nov-Dec;44(6):8S.

Additional information
regarding the Genome Project

Cushion MT and Smulian AG. Pneumocystis Genome Project.
In Pneumocystis pneumonia, Walzer PD and Cushion MT, eds. 3rd ed. (in press)

Sesterhenn TM, Slaven BE, Smulian AG, Cushion MT. Generation of sequencing libraries for the Pneumocystis Genome project.
J Eukaryot Microbiol. 2003;50 Suppl:663-5.

Xu Z, Lance B, Vargas C, Arpinar B, Bhandarkar S, Kraemer E, Kochut KJ, Miller JA, Wagner JR, Weise MJ, Wunderlich JK, Stringer J, Smulian G, Cushion MT, Arnold J. Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool.
Genetics. 2003 Apr; 163(4):1299-313.

Cushion MT, Smulian AG. The Pneumocystis genome project: update and issues.
J Eukaryot Microbiol. 2001; Suppl:182S-183S.

Information on
the genome of Pneumocystis

Cushion MT. Pneumocystis: unraveling the cloak of obscurity.
Trends Microbiol. 2004 May;12(5):243-9. Review.

Cushion MT. Comparative genomics of Pneumocystis carinii with other protists: implications for life style.
J Eukaryot Microbiol. 2004 Jan-Feb;51(1):30-7. Review.

Cornillot E, Keller B, Cushion MT, Metenier G, Vivares CP. Fine analysis of the Pneumocystis carinii f. sp. carinii genome by two-dimensional pulsed-field gel electrophoresis.
Gene. 2002 Jun 26;293(1-2):87-95.

Stringer JR, Cushion MT. The genome of Pneumocystis carinii.
FEMS Immunol Med Microbiol. 1998 Sep;22(1-2):15-26. Review.

Citation form

If you use any sequence information from this website, please use the following manner of reference in any publication: "These sequence data were produced by the Pneumocystis Genome Project (funded by the NIH NIAID) and can be obtained from http://pgp.cchmc.org".

If you use any textual information, please cite the source as: "The Pneumocystis Genome Project. http://pgp.cchmc.org".