The goals of this project are to provide a physical map,
gene sequence, and an expressed gene inventory for
Pneumocystis carinii as a means to better understand
their molecular structure and develop publicly available,
centralized information and reagents that will be used to
expedite research. This project will accomplish the following
specific goals over a 5 year period starting February 15, 1999
through February 14, 2005:
Construct cosmid libraries of the rat P. carinii
genome in at least 2 vector systems.
Sequence cDNAs of rat Pc for creation of expression sequence
Create chromosome-specific libraries.
Order the clones containing the rat P. carinii
DNA along the chromosome of origin to create an in vitro
reconstruction of each chromosome.
Sequence the entire genome of P. carinii.
Perform concurrent functional genomic studies to address basic
biological questions in Pneumocystis research.